Y-27632 reduces the MMP2 and MMP9 expression in endothelial cell via inhibition of ROCK signal pathway / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 566-570, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-814997
ABSTRACT
OBJECTIVE@#To explore the effect of ROCK inhibitor Y-27632 on the matrix metalloproteinase 2 and 9 (MMP2 and MMP9) gene expression and activity in tumor necrosis factor α (TNF-α)-treated human umbilical vein endothelial cell (HUVEC).
@*METHODS@#HHUVEC was divided into 3 groups, a control group, a TNF-α group, and a TNF-α plus Y-27632 group. The expressions of vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), MMP2 and MMP9 were examined by real-time PCR. The MMP2/9 activity was measured by gelatin zymography.
@*RESULTS@#Compared to the control group, the mRNA expressions of ICAM-1, VCAM-1, MMP2 and MMP9 were increased TNF-α-treated cells, which were suppressed by ROCK inhibitor (P<0.01). The MMP2/9 activity was elevated in TNF-α-treated cells, which was reversed by ROCK inhibitor (P<0.05).
@*CONCLUSION@#ROCK inhibitor can suppress TNF-α-induced inflammation in endothelial cells through down-regulation of MMP2/9.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pyridines
/
Umbilical Veins
/
Signal Transduction
/
Down-Regulation
/
Cells, Cultured
/
Tumor Necrosis Factor-alpha
/
Intercellular Adhesion Molecule-1
/
Vascular Cell Adhesion Molecule-1
/
Matrix Metalloproteinase 2
/
Matrix Metalloproteinase 9
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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