Expression of two transcript variants of long noncoding RNA C6orf176 in non-small cell lung cancer and its clinical significance / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 560-565, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-814998
ABSTRACT
OBJECTIVE@#To explore two transcript variants expression of long noncoding RNA C6orf176 in non-small cell lung cancer (NSCLC) and the clinical pathological significance.
@*METHODS@#The expressions of transcript variant 1 (TV1) and transcript variant 2 (TV2) of long noncoding RNA C6orf176 in 57 NSCLC and adjacent cancerous tissues were examined by qPCR with β-actin as internal control.
@*RESULTS@#Based on the results of qPCR, for C6orf176-TV1, 42 cases were down-regulated and 15 cases were up-regulated. The C6orf176-TV1 level was correlated to the grade of differentiation (P<0.05) but it was not correlated with gender, age, smoking history, tumor type and TNM stage. The expression of C6orf176-TV1 had a potential value in diagnosis of NSCLC by receiver operator characteristic (ROC) curve. Area under curve (AUC) of ROC curve was 0.708 (95% CI 0.615 to 0. 802). The sensitivity and specificity were 51% and 88%, respectively. For C6orf176-TV2, 39 cases were down-regulated and 18 cases were up-regulated. The C6orf176-TV2 level was correlated with the grade of differentiation (P<0.05) but it was not correlated with gender, age, tumor size, smoking history and TNM stage. C6orf176-TV2 level had value in diagnosis of NSCLC by ROC curve. AUC of ROC curve was 0.64 (95% CI 0.531 to 0.749). The sensitivity and specificity were 49% and 75%, respectively. Of the 57 specimens, 53 cases were simultaneous up or down-regulation of C6orf176-TV1 and C6orf176-TV2. The correlation coefficient was 0.99.
@*CONCLUSION@#The expression of C6orf176-TV1 or C6orf176-TV2 is down-regulated in NSCLC and it is correlated with the grade of differentiation. It may act as a diagnosis indicator for NSCLC patients.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transcription, Genetic
/
Smoking
/
Down-Regulation
/
Gene Expression Regulation, Neoplastic
/
Cell Differentiation
/
Up-Regulation
/
ROC Curve
/
Actins
/
Carcinoma, Non-Small-Cell Lung
/
Area Under Curve
Type of study:
Prognostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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