Effect of hydrogen sulfide on cardiac myosin light chain kinase expression in diabetic rats / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 353-358, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-815030
ABSTRACT
OBJECTIVE@#To investigate the effect of hydrogen sulfide (H2S) on cardiac myosin light chain kinase (MLCK) expression in diabetic rats.
@*METHODS@#A total of 32 male SD rats were randomly divided into a normal control group (NC group), a diabetic control group (DM), a NaHS treatment group (DM+NaHS) and a NaHS group (NaHS) (n=8 in each group). Intraperitoneal injection of streptozotocin was utilized to establish Type 1 diabetic rat model. The diabetic rats in the DM+NaHS and NaHS groups were intraperitoneally injected with 28 μmol/kg NaHS solution. Eight weeks later, the ventricular hemodynamic parameters, the ratio of heart weight/body weight (HW/BW ratio), the levels of lactate dehydrogenase (LDH) and creatine kinase MB isozyme (CK-MB) in serum were determined. The ultrastructures of myocardium were observed under electron microscopy. The expressions of MLCK mRNA and protein level in myocardium were detected by RT-PCR and Western blot, respectively.
@*RESULTS@#Compared with the NC group, there was no significant difference in the various indexes in the NaHS group (all P>0.05). The function of left ventricular contract and relaxation were decreased obviously in diabetic rats, while the HW/BW ratio was increased (all P<0.01). The levels of LDH and CK-MB were increased (both P<0.01) in serum, while the levels of MLCK mRNA and protein were decreased significantly (both P<0.01) in myocardial tissues. Compared with the DM group, the left ventricular hemodynamic parameters and myocardial ultrastructure damage were improved in the DM+NaHS group, while the HW/BW ratio was decreased (all P<0.05). The levels of LDH and CK-MB were decreased (both P<0.01), while the levels of MLCK mRNA and protein were increased significantly (both P<0.01).
@*CONCLUSION@#H2S can protect myocardium in diabetic rats, which may be associated with upregulation of cardiac MLCK.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Sulfides
/
Blood
/
Myosin-Light-Chain Kinase
/
Cardiotonic Agents
/
Random Allocation
/
Ventricular Function, Left
/
Rats, Sprague-Dawley
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Diabetes Mellitus, Experimental
/
Drug Therapy
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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