Mechanisms for effect of osthole on inhibiting the growth and invasion of bladder cancer cells / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 345-352, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-815031
ABSTRACT
OBJECTIVE@#To investigate the effect of osthole on epidermal growth factor receptor tyrosine kinase (EGFR-TPK), matrix-metalloproteinase-2 (MMP-2), aminopeptidase N (APN) in bladder cancer cell and the underlying mechanism.
@*METHODS@#The T24 cell lines were cultured. The inhibitory effects of osthole on EGFR-TPK, APN and MMP-2 were evaluated by spectrophotometric and MTT assay. The caspase-3 activity and the expression COX-2 and VEGF in T24 were examined. The activity of NF-κB was determined by electrophoretic mobility shift assay.
@*RESULTS@#The half inhibition concentrations (IC50) of osthole on EGFR-TPK, APN and MMP-2 were (45.33±3.98), (28.21±3.23) and (8.11±0.54) µmol/L, respectively. The growth inhibitory rates for T24 cells were increased in a dose-dependent manner (P<0.05). The caspase-3 activities were significantly increased in T24 cells in the osthole group compared with control group, while the expression of angiogenesis related-protein COX-2, VEGF, and NF-κB in T24 cells were decreased.
@*CONCLUSION@#Through the inhibitory effect on EGFR-TPK, APN and MMP-2, osthole can decrease COX-2, VEGF and NF-κB expression while increase the activity of caspase-3, eventually blocking the growth and invasion of bladder cancer cell.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Urinary Bladder Neoplasms
/
NF-kappa B
/
CD13 Antigens
/
Coumarins
/
Matrix Metalloproteinase 2
/
Cell Line, Tumor
/
Vascular Endothelial Growth Factor A
/
Cyclooxygenase 2
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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