Mechanism of inhibitory effects of silencing high mobility group box-1 on invasion and migration of endometrial carcinoma of uterus / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To determine the effects of p38 mitogen-activated protein kinase (MAPK) signal pathway after small hairpin RNA on inhibiting the expression of high mobility group box-1 (HMGB1), and to explore the mechanism responsible for the effects of HMGB1 on invasion and migration of endometrial carcinoma of uterus.
@*METHODS@#Based on RNA interference technology, we designed and synthetized HMGB1 small hairpin RNA (HMGB1 shRNA-1, HMGB1 shRNA-2, HMGB1 shRNA-3, HMGB1 shRNA-1), and then transfected HMGB1 shRNA plasmids into HEC-1A cells. The expression of genes and proteins was examined by RT-PCR and Western blot. The activity of HEC-1A cells was evaluated by the methyl thiazolyl tetrazolium (MTT) method.
@*RESULTS@#HMGB1 shRNA effectively inhibited the expression of the HMGB1 and p38MAPK in HEC-1A cells (P<0.05). Among the shRNAs, the inhibitory effect of HMGB1 shRNA-3 was the best (P<0.05). According to the results of MTT, the growth of HEC-1A cells was obviously inhibited after transfection of HEC-1A HMGB1 shRNA (P<0.05). 
@*CONCLUSION@#Downregulation of HMGB1 may effectively inhibit proliferation, invasion and migration of HEC-1A cells in endometrial carcinoma of uterus, which is dependent on p38MAPK signal pathway.