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A study on the transfection of antisense oligonucletide into kidney mediated by lipid microbubbles / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 113-120, 2016.
Article in English | WPRIM | ID: wpr-815065
ABSTRACT
OBJECTIVE@#To study the safety and efficiency of the transfection of antisense oligonucletide into kidney mediated by lipid microbubbles, and to evaluate its potential clinical application.@*METHODS@#The potential and conditions regarding the transfection self-made lipid microbubbles (CY5)-labeled-oligonucleotide (ODN) or CY5-labeled-ODN connective tissue growth factor (CTGF) into the rat kidney were evaluated. Th e safety was evaluated by HE staining, liver and renal function tests. The transfection efficiency was evaluated by fluorescence microscopy. Th e expression of CTGF was detected by RT-PCR and Western blot.@*RESULTS@#Self-made lipid microbubble and/or ultrasound significantly enhanced the efficiency of gene transfer and expression in the kidney. Especially, 85%-90% of total glomerular could be transfected. CY5-labeled-ODN expression could be observed in glomerular, tubular and interstitial area. Th ere was no significant change in blood tests aft er gene transfer. Levels of LDH in 7 days were decreased compared with that at the fi rst day aft er the transfection (P<0.05). CTGF expression was successfully suppressed by transfection of CTGF-antisense-ODN into kidney.@*CONCLUSION@#The ultrasound-mediated gene transfer by self-made lipid microbubble could enhance the efficiency of ODN and expression in the rat kidney. Th is self-made lipid microbubbles supplement may be use for transfection of target genes.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Ultrasonics / RNA, Messenger / Transfection / Chemistry / Oligonucleotides, Antisense / Microbubbles / Connective Tissue Growth Factor / Genetics / Kidney / Lipids Limits: Animals Language: English Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Ultrasonics / RNA, Messenger / Transfection / Chemistry / Oligonucleotides, Antisense / Microbubbles / Connective Tissue Growth Factor / Genetics / Kidney / Lipids Limits: Animals Language: English Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article