A study on the transfection of antisense oligonucletide into kidney mediated by lipid microbubbles / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 113-120, 2016.
Article
in English
| WPRIM
| ID: wpr-815065
ABSTRACT
OBJECTIVE@#To study the safety and efficiency of the transfection of antisense oligonucletide into kidney mediated by lipid microbubbles, and to evaluate its potential clinical application.@*METHODS@#The potential and conditions regarding the transfection self-made lipid microbubbles (CY5)-labeled-oligonucleotide (ODN) or CY5-labeled-ODN connective tissue growth factor (CTGF) into the rat kidney were evaluated. Th e safety was evaluated by HE staining, liver and renal function tests. The transfection efficiency was evaluated by fluorescence microscopy. Th e expression of CTGF was detected by RT-PCR and Western blot.@*RESULTS@#Self-made lipid microbubble and/or ultrasound significantly enhanced the efficiency of gene transfer and expression in the kidney. Especially, 85%-90% of total glomerular could be transfected. CY5-labeled-ODN expression could be observed in glomerular, tubular and interstitial area. Th ere was no significant change in blood tests aft er gene transfer. Levels of LDH in 7 days were decreased compared with that at the fi rst day aft er the transfection (P<0.05). CTGF expression was successfully suppressed by transfection of CTGF-antisense-ODN into kidney.@*CONCLUSION@#The ultrasound-mediated gene transfer by self-made lipid microbubble could enhance the efficiency of ODN and expression in the rat kidney. Th is self-made lipid microbubbles supplement may be use for transfection of target genes.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Ultrasonics
/
RNA, Messenger
/
Transfection
/
Chemistry
/
Oligonucleotides, Antisense
/
Microbubbles
/
Connective Tissue Growth Factor
/
Genetics
/
Kidney
/
Lipids
Limits:
Animals
Language:
English
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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