Hypoxic condition promotes olfactory mucosa mesenchymal stem cells to differentiate into neurons and
underlying mechanisms / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1252-1259, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-815100
ABSTRACT
To explore whether hypoxic condition could promote the olfactory mucosa mesenchymal stem cells (OM-MSCs) to differentiate into neurons with the olfactory ensheathing cells (OECs) supernatant and the potential mechanisms.
Methods:
The OM-MSCs and OECs were isolated and cultured, and they were identified by flow cytometry and immunofluorescence. The OM-MSCs were divided into three groups a 3%O2+ HIF-1α inhibitors (lificiguat YC-1) + OECs supernatant group (Group A) , a 3%O2 + OECs supernatant group (Group B) and a 21%O2 + OECs supernatant group (Control group). The neurons, which were differentiated from OM-MSCs, were assessed by immunofluorescence test. The mRNA and protein expression of hypoxia-inducible factor-1α (HIF-1α), βIII-tubulin and glial fibrillary acidic portein (GFAP) were detected by quantitative polymerase chain reaction (Q-PCR) and Western blot. The potassium channels were analyzed by patch clamp.Results:
The neurons differentiated from OM-MSCs expressed the most amount of βIII-tubulin, and the result of Q-PCR showed that HIF-1α expression in the Group B was significantly higher than that in the other groups (all P<0.05). Western blot result showed that the βIII-tubulin protein expression was significantly higher and GFAP protein expression was obviously decreased in the Group B (both P<0.05). The patch clamp test confirmed that the potassium channels in the neurons were activated.Conclusion:
Hypoxic condition can significantly increase the neuronal differentiation of OM-MSCs by the OECs supernatant and decrease the production of neuroglia cells, which is associated with the activation of HIF-1 signal pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Physiology
/
Tubulin
/
Potassium Channels
/
Signal Transduction
/
Cell Differentiation
/
Cells, Cultured
/
Chemistry
/
Olfactory Mucosa
/
Neuroglia
Type of study:
Prognostic study
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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