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Hypoxic condition promotes olfactory mucosa mesenchymal stem cells to differentiate into neurons and 
underlying mechanisms / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 1252-1259, 2016.
Article in Chinese | WPRIM | ID: wpr-815100
ABSTRACT
To explore whether hypoxic condition could promote the olfactory mucosa mesenchymal stem cells (OM-MSCs) to differentiate into neurons with the olfactory ensheathing cells (OECs) supernatant and the potential mechanisms.


Methods:

The OM-MSCs and OECs were isolated and cultured, and they were identified by flow cytometry and immunofluorescence. The OM-MSCs were divided into three groups a 3%O2+ HIF-1α inhibitors (lificiguat YC-1) + OECs supernatant group (Group A) , a 3%O2 + OECs supernatant group (Group B) and a 21%O2 + OECs supernatant group (Control group). The neurons, which were differentiated from OM-MSCs, were assessed by immunofluorescence test. The mRNA and protein expression of hypoxia-inducible factor-1α (HIF-1α), βIII-tubulin and glial fibrillary acidic portein (GFAP) were detected by quantitative polymerase chain reaction (Q-PCR) and Western blot. The potassium channels were analyzed by patch clamp.


Results:

The neurons differentiated from OM-MSCs expressed the most amount of βIII-tubulin, and the result of Q-PCR showed that HIF-1α expression in the Group B was significantly higher than that in the other groups (all P<0.05). Western blot result showed that the βIII-tubulin protein expression was significantly higher and GFAP protein expression was obviously decreased in the Group B (both P<0.05). The patch clamp test confirmed that the potassium channels in the neurons were activated.


Conclusion:

Hypoxic condition can significantly increase the neuronal differentiation of OM-MSCs by the OECs supernatant and decrease the production of neuroglia cells, which is associated with the activation of HIF-1 signal pathway.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Tubulin / Potassium Channels / Signal Transduction / Cell Differentiation / Cells, Cultured / Chemistry / Olfactory Mucosa / Neuroglia Type of study: Prognostic study Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Physiology / Tubulin / Potassium Channels / Signal Transduction / Cell Differentiation / Cells, Cultured / Chemistry / Olfactory Mucosa / Neuroglia Type of study: Prognostic study Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article