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Effect of prolonged propofol infusion on myocardial enzyme, mitochondrial cytochrome C and adenosine 
triphosphate in rabbits / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 1181-1185, 2016.
Article in Chinese | WPRIM | ID: wpr-815113
ABSTRACT
To explore the effect of long-time propofol infusion on myocardial enzymes, mitochondrial cytochrome C and ATP in rabbits. 


Methods:

A total of 18 New Zealand rabbits were randomly divided into 3 groups a control group, a propofol group and an intralipid group. The rabbits were continuously infused with 0.9% normal saline in the control group, 1% propofol in the propofol group, and 10% intralipid in the intralipid group, respectivey. The arterial blood was collected at 0, 8, 16 h and the end of experiment to examine creatine kinase (CK) and creatine kinase isoenzyme (CK-MB). In the end, the myocardial mitochondria from myocardial tissues was separated by differential centrifugation, and mitochondrial cytochrome C content and adenosine triphosphate (ATP) levels were examined by high performance liquid chromatography.


Results:

Compared with the control group, the release of cytochrome C from mitochondria were increased in the propofol group and the intralipid group (both P0.05). There was also no significant difference in the ATP content of the mitochondria among the 3 groups (P>0.05). The levels of CK were increased at 8, 16 and 24 h after infusion in the propofol group and the intralipid group compared with that before the infusion (all P0.05); compared with the control group, the levels of CK-MB were obviously increased in the infusion of propofol for 24 h in the propofol group (P<0.05).


Conclusion:

The levels of serum CK increase after the infusion of propofol and intralipid for a long time, and the levels of CK-MB also elevate in the infusion of propofol. Propofol and intralipid can increase the release of myocardial mitochondrial cytochrome C, but they don't affect the ATP production in myocardial mitochondrial.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phospholipids / Polyphosphates / Blood / Infusions, Intravenous / Soybean Oil / Propofol / Adenosine Triphosphate / Chemistry / Creatine Kinase Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pharmacology / Phospholipids / Polyphosphates / Blood / Infusions, Intravenous / Soybean Oil / Propofol / Adenosine Triphosphate / Chemistry / Creatine Kinase Limits: Animals Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2016 Type: Article