Identification of interacting proteins with NF-κB in
different status of uterine smooth muscle in labor / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1039-1046, 2016.
Article
in Chinese
| WPRIM
| ID: wpr-815136
ABSTRACT
To analyze the differentially expressed proteins which interacted with NF-kappaB in the uterine lower segment smooth muscle tissues under different status of labor onset, and to provide a new foundation on the mechanisms for labor onset.
Methods:
NF-κB P65 protein expression in smooth muscle tissues from the term non-labor group, natural term labor group and drug-induced term labor group was analyzed by Western blot. Co-immunoprecipitation and SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) were performed to detect the proteins interacting with NF-κB p65 in the NF-κB p65 complexes. The components of the complex were identified by LC-ESI-MS/MS (liquid chromatography-tandem electrospray mass spectrometry) and database analysis. The identified differentially expressed proteins were confirmed by Western blot.Results:
Positive expression of NF-κB was detected in all of the three groups. 10 differentially expressed proteins were identified by LC-ESI-MS/MS in human lower segment myometrium tissues in the term non-labor group and natural term labor group, mean while, 5 differentially expressed proteins were identified in the term non-labor group and the drug-induced labor group. 3 differential expression proteins were detected in all of the 3 groups, including Heat shock 70, Annexin A6 and Desmin, which were verified by Western blot. These proteins were mainly involved in chaperone, signal transduction, cell structure, and energy metabolism process, respectively.Conclusion:
NF-κB expressed in uterine smooth muscle cells is involved in the process of initiation and regulation of labor onset through a number of proteins relevant to signal transduction, cell structure and energy metabolism.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Labor, Obstetric
/
Signal Transduction
/
Blotting, Western
/
NF-kappa B
/
Molecular Chaperones
/
Protein Interaction Mapping
/
Myocytes, Smooth Muscle
/
Proteomics
/
Immunoprecipitation
Type of study:
Diagnostic study
/
Prognostic study
Limits:
Female
/
Humans
/
Pregnancy
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2016
Type:
Article
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