Protective effect of heart-fatty acid binding protein on lipopolysaccharide-induced cardiomyocyte damage / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 457-463, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-815148
ABSTRACT
OBJECTIVE@#To observe the protective effect of heart-fatty acid binding protein (H-FABP) on lipopolysaccharide (LPS)-induced cardiomyocyte damage.@*METHODS@#The cardiomyocytes were isolated and cultured from 1-3 days old neonatal rats. The specific siRNA or plasmid of H-FABP were transfected into cells to alter H-FABP expression, which was evaluated by Western blot and quantitative-PCR. LPS-induced cardiomyocyte damage and inflammation were estimated by detecting the contents of lactate dehydrogenase(LDH), TNF-α, and IL-1β as well as cell viability.@*RESULTS@#LPS treatment induced inflammation and cell damage indicated by a decrease in cell viability and an increase in LDH, TNF-α and IL-1β in the medium. When H-FABP was downregulated by siRNA transfection, the LPS-induced inflammation and cell damage were augmented. In contrast, when H-FABP was overexpressed by pcDNA3.1-H-FABP transfection, the LPS-induced inflammation and cell damage were suppressed.@*CONCLUSION@#H-FABP protects cardiomyocytes from LPS-induced inflammation and cell injury.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Transfection
/
Down-Regulation
/
Cell Line
/
Cell Survival
/
Lipopolysaccharides
/
Tumor Necrosis Factor-alpha
/
Cell Biology
/
Myocytes, Cardiac
/
RNA, Small Interfering
/
Fatty Acid-Binding Proteins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article
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