Effect of Par-4 on the apoptosis of islet β cell / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 6-11, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-815213
ABSTRACT
OBJECTIVE@#To explore the effect of high glucose and lipid intervention on islet cell apoptosis through the inhibition of prostate apoptosis response factor-4 (Par-4) expression and the underlying mechanisms.@*METHODS@#The mice islet β cells (NIT-1 cells) were randomly divided into a control group, a Par-4 inhibited group, a glucose and fatty acid intervented group and a glucose and fatty acid intervented+Par-4 inhibited group. Cell apoptosis was detected by terminal dexynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL), and the protein expression levels of Par-4 and glucose regulated protein 78 (GRP78) were detected by Western blot.@*RESULTS@#Compared with the control group [(3.14 ± 1.08)%], the apoptosis rate of islet beta cell [(33.82 ± 3.15)%] in the glucose and fatty acid intervented group was significantly increased accompanied by the dramatically elevated Par-4 and GRP78 expression (both P<0.05). Compared with the glucose and fatty acid intervented group, the apoptosis rate in glucose and fatty acid intervented+Par-4 inhibit group [(18.3 4 ± 2.11)%] was significantly decreased concomitant with the significantly decreased Par-4 and GRP78 expression (both P<0.05).@*CONCLUSION@#The glucose and fatty acid-induced apoptosis of mice islet β cells could be improved through the inhibition of Par-4 expression, which might be related to reduction of endoplasmic reticulum stress.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Cell Line
/
Gene Expression Regulation
/
Islets of Langerhans
/
Apoptosis
/
In Situ Nick-End Labeling
/
Cell Biology
/
Apoptosis Regulatory Proteins
/
Fatty Acids
/
Glucose
/
Heat-Shock Proteins
Limits:
Animals
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article
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