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Grape seed proanthocyanidins extract inhibits pancreatic cancer cell growth through down-regulation of miR-27a expression / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences) ; (12): 46-52, 2015.
Article in Chinese | WPRIM | ID: wpr-815215
ABSTRACT
OBJECTIVE@#To explore the eff ect of grape seed proanthocyanidins extract (GSPE) on the growth of pancreatic cancer cells and the underlying mechanisms.@*METHODS@#The pancreatic cancer AsPC-1 cells were cultured in vitro. The effects of GSPE on cell proliferation, apoptosis and migration were analyzed by MTT, Annexin V-FITC/PI and Transwell migration assay, respectively. The expression of miR-27a and FOXO1 in AsPC-1 cells was determined by real-time RT-PCR and Western blot, respectively. The miR-27a inhibitors were applied to verify the role of miR-27a in mediation of GSPE effects.@*RESULTS@#GSPE inhibited cell growth in a dose-dependent manner. This inhibitory effect was significant when the dosage of GSPE was more than 50 μg/mL (P<0.05 vs control). GSPE also could induce apoptosis and inhibit cell migration. MiR-27a expression was notably down-regulated when the dosage of GSPE was 75 μg/mL (P<0.01 vs control). Compared with the control group, cell proliferation inhibition was significantly increased in the miR-27a inhibitor group, the GSPE group and the miR-27a inhibitor plus GSPE group (P<0.01), while cell migration was significantly decreased (P<0.01). Compared with the GSPE or the miR-27a inhibitor group, the growth and migration inhibitory effects in the miR-27a inhibitor plus GSPE group were more obviously (P<0.01). Both GSPE and miR-27a inhibitor alone could up-regulate FOXO1 expression. But these effects were more apparent when they are applied in combination.@*CONCLUSION@#GSPE inhibites AsPC-1 cells' growth and migration partly through down-regulation of miR-27a expression.
Subject(s)
Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreatic Neoplasms / Pathology / Pharmacology / Down-Regulation / Up-Regulation / Cell Movement / Apoptosis / MicroRNAs / Cell Line, Tumor / Proanthocyanidins Limits: Humans Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2015 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Main subject: Pancreatic Neoplasms / Pathology / Pharmacology / Down-Regulation / Up-Regulation / Cell Movement / Apoptosis / MicroRNAs / Cell Line, Tumor / Proanthocyanidins Limits: Humans Language: Chinese Journal: Journal of Central South University(Medical Sciences) Year: 2015 Type: Article