Ultrasound molecular detection of immediately blood-mediated inflammatory reaction induced by
islets transplantation in vitro / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 632-638, 2015.
Article
in Chinese
| WPRIM
| ID: wpr-815295
ABSTRACT
OBJECTIVE@#To study the feasibility of ultrasonic molecular imaging of immediately blood-mediated inflammatory reaction (IBMIR) in vitro.
@*METHODS@#IBMIR models in vitro were divided into 3 groups Group A, no microbubbles were added; Group B, non-targeted micro-bubbles were added; Group C, Lys-Gly-Asp-Ser (KGDS)-targeted microbubbles (MBK) were added. The ultrasonic enhancement of IBMIR in loops by ultrasonic contrast imaging was evaluated.
@*RESULTS@#The contrast-enhanced US imaging did not show thrombus formation in the group A, whereas the thrombus was found in the Group B and C with a change in filling defects or ring enhancement, respectively. The time for detecting thrombosis was (7.3 ± 0.5) min and (13.2 ± 0.6) min in Group B and Group C, respectively (P<0.05). The average-gray scales of thrombus in Group B and Group C were 31.22 ± 3.56 and 75.85 ± 5.21, respectively (P<0.05). The fluorescence microscope also showed that MBK was attached to thrombus surrounding islets.
@*CONCLUSION@#IBMIR model in vitro showed that KGDS-targeted ultrasound contrast agent could adhere to thrombus shell surrounding islets and molecular target ultrasonography could image these thrombi noninvasively and effectively.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Thrombosis
/
Diagnostic Imaging
/
Islets of Langerhans Transplantation
/
Ultrasonography
/
Contrast Media
/
Microbubbles
/
Inflammation
Type of study:
Diagnostic study
Limits:
Humans
Language:
Chinese
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article
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