L10P mutation in DJ-1 gene induced oxidative stress and mitochondrial disfunction / 中南大学学报(医学版)
Journal of Central South University(Medical Sciences)
;
(12): 1285-1291, 2015.
Article
in English
| WPRIM
| ID: wpr-815339
ABSTRACT
OBJECTIVE@#To investigate the effect of the L10P mutation on the cellular mitochondrial disfunction.@*METHODS@#Spectrophotometer, flow cytometry and electron microscope was utilized to examine cell viability, reactive oxygen species (ROS), mitochondrial transmembrane potential, complex I activity and mitochondrial morphous of the HEK293 monoclone cell lines, in which wild-type and L10P mutant DJ-1 protein are stably expressed.@*RESULTS@#Compared with the cell lines expressing empty vector, we found the ROS levels were elevated, the cell viability, mitochondrial transmembrane potential, complex I activity were reduced in the cells expressing L10P mutant DJ-1 protein (P<0.05). We also found mitochondria in these cells were swelling and some mitochondria were vacuolar degeneration. These phenomena were more obvious when rotenone was used. But in the cells expressing wild-type DJ-1, ROS levels were lower, the cell viability, mitochondrial transmembrane potential, and complex I activity were higher than other cell lines (P<0.05), especially under the induction of rotenone. These results suggested that L10P mutant DJ-1 protein probably lost the ability of anti-oxidative stress and affect the normal function of mitochondria.@*CONCLUSION@#The L10P DJ-1 mutation results in a toxic protein, which lacks the protective function of wild-type protein on mitochondria due to the decrease in the ability of anti-oxidative stress.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Rotenone
/
Cell Survival
/
Reactive Oxygen Species
/
Oncogene Proteins
/
Oxidative Stress
/
Intracellular Signaling Peptides and Proteins
/
Membrane Potential, Mitochondrial
/
HEK293 Cells
/
Protein Deglycase DJ-1
Limits:
Humans
Language:
English
Journal:
Journal of Central South University(Medical Sciences)
Year:
2015
Type:
Article
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