Different stimulant intensity of abdominal operation on the expression of tumor necrosis factor α and surfactant protein-A in lung of the rats / 中南大学学报(医学版)

ABSTRACT

OBJECTIVE@#To determine the influence of different stimulation of abdominal operation on lung.@*METHODS@#We randomly divided 100 SD male rats into 5 groups(n=20) An anesthesia control group (Group A), an abdominal skin 2 cm incision group (Group B), an abdominal skin+ muscle 2 cm incision group (Group C ), an abdominal skin+muscle+peritoneum 2 cm incision group (Group D), and an abdominal skin+muscle+peritoneum 2 cm incision+2 min laparoscopy in abdomen+pull exploration bowel group (Group E). Propofol was used for induction of anaesthesia by rat tail vein with 10 mg/kg, with 35-45 mg/(kg.h) during the operation. Anesthesia depth was maintained during moderate sedation without obvious analgesia action level. According to the postoperative specimens at different time, each group was divided into 4 sub groups, including 6-hour group, 1-day group, 3-day group, and 7-day group(n=5). Immunohistochemical method was used to examine tumor necrosis factor (TNF-α) and lung tissue lung surface active substances related proteins A (SP-A) of rats at different points after surgery.@*RESULTS@#There was no significant differences in the expression of TNF-α at 6 hours, 3 days, and 7 days after surgery in the 5 groups (P>0.05). The expression of TNF-α in Group D, and E was significantly higher than that in Group A at 1 day after surgery (P Group D > Group C > Group B at 1 day, 3 days after surgery, there was no significant difference among the 4 groups. There was no significant differences in the expression of SP-A at 6 hours, 3 days, and 7 days after surgery in the 5 groups (P>0.05). The expression of SP-A in Group D and E was significantly higher than that in Group A at 1 day after surgery (P0.05).@*CONCLUSION@#The greater the stimulant intensity of abdominal operation on rats, the more impact on postoperative lung. The postoperative pulmonary effect of intraperitoneal operation is greater than non-intraperitoneal operation, but in the compensation.