The establishment of Kunming mouse models of lung tumor / 预防医学

ABSTRACT

Objective@#To establish Kunming mouse models of lung tumor rapidly induced by urethane and butylated hydroxytoluene（BHT）.@*Methods@#Two hundred Kunming mice of SPF grade were randomly assigned to one control group and three experimental groups. The mice in the experimental groups were intraperitoneally injected with urethane at the dosage of 50,100 and 200 mg/kg（low,medium and high dose）,after one week,they were fed with fodder containing 0.75% BHT every day for two weeks;while the mice in the control group were injected with distilled water and were fed with normal fodder without BHT. At the 15th week,the lungs of all the mice were removed,tumors were counted and collected. The remaining lung tissue was cut into blocks of 1-2 mm thick,followed by paraffin section,HE staining,and was observed under light microscope.@*Results@#The incidence rates of lung tumors in Kunming mice of the low-,meidium-,high-dose group and the control group were 14.00%,24.00%,38.00% and 2.00%;the number of tumors were 1,8,22 47;the proportion of malignant tumors were 12.50%,18.18%,27.66% and 0. The incidence rate and the number of tumors in medium- and high-dose group were significantly higher than those in the control group（P<0.05）. The incidence rate,the number of tumors and the proportion of malignant tumors increased with the dosage of urethane,which indicated a dose-response relationship（P<0.05）.@*Conclusion@#The Kunming mouse models of lung tumor rapidly induced by urethane and BHT were established successfully,with significant dose-response relationship and tumorigenesis.