Stable Expression of Bovine Integrin Beta-6 Increases Susceptibility of Goat Kidney Cell Line to Foot-and-mouth Disease Virus
Journal of Bacteriology and Virology
;
: 35-43, 2020.
Article
in Korean
| WPRIM
| ID: wpr-816638
ABSTRACT
The integrins αvβ1, αvβ3, αvβ6, and αvβ8 are known to be the natural receptors of foot-and-mouth disease virus (FMDV). Among them, integrin αvβ6 is considered a major receptor for FMDV. We performed protein expression of full-length bovine integrins αv, β3, and β6 and confirmed the high efficiency of bovine αvβ6 as the FMDV receptor in FMDV non-permissive SW 480 cells. Next, we established the black goat kidney (BGK) cell line, stably expressing bovine integrin β6 (BGK-β6-4). We observed that BGK-β6-4 cells had significantly enhanced sensitivity to FMDV compared with that of BGK cells (P<0.05). In addition, BGK-β6-4 cells had equal or higher sensitivity to several serotypes of FMDV compared with that of other FMDV permissive cell lines, such as BHK-21 and IBRS-2. In conclusion, we established a promising novel goat cell line, BGK-β6-4, which can be used to isolate or culture FMDV. Furthermore, the BGK-β6-4 cell line may serve as a promising tool for studying integrin αvβ6 receptor functions.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Goats
/
Integrins
/
Cell Line
/
Foot-and-Mouth Disease Virus
/
Serogroup
/
Foot-and-Mouth Disease
/
Kidney
Limits:
Animals
Language:
Korean
Journal:
Journal of Bacteriology and Virology
Year:
2020
Type:
Article
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