Study on the Effects of Tetrandrine on Proliferation and Migration ，Invasion Ability of Neuroblastoma Cells and Its Mechanism / 中国药房

ABSTRACT

OBJECTIVE： To investigate the effects of tetrandrine （TET） on the proliferation and migration， invasion ability of neuroblastoma cells and its mechanism. METHODS： Human neuroblastoma cell lines IMR-32 and SH-SY5Y were chosen as objects. MTT assay was used to detect the effects of 0 （blank control）， 2.5， 5， 10， 15， 20 μmol/L TET on cell proliferation. The transmembrane number of normal control group （TET concentration of 0 μmol/L） and TET group （10 μmol/L for IMR-32 cells， 15 μmol/L for SH-SY5Y cells） were investigated by Transwell cell migration and invasion experiments. The protein levels of MMP-2， MMP-9， β-catenin， GSK3β and p-GSK3β in IMR-32 cells and SH-SY5Y cells were tested by Western blotting assay after treated with TET of above concentrations. RESULTS： TET with 5， 10， 15， 20 μmol/L can reduce the survival rate of IMR-32 cells and SH-SY5Y cells significantly （P＜0.05 or P＜0.01）. IC50 of which to IMR-32 cells and SH-SY5Y cells were 10.148 and 14.461 μmol/L， respectively. Results of migration and invasion experiments showed that compared with normal control group， the number of transmembrane cells was decreased significantly in TET group （P＜0.01）. Results of Western blotting assay showed that compared with normal control group， the protein expression levels of MMP-2，MMP-9，β-catenin and p-GSK3β were decreased significantly in TET group （P＜0.01）. CONCLUSIONS： TET shows significant inhibitory effects on the proliferation， migration and invasion ability of neuroblastoma cells， the mechanism of which may be associated with down-regulating the protein expression of MMP-2 and MMP-9 and inhibiting Wnt/β-catenin signaling pathway.