Determination of Fluconazole in Human Plasma by UPLC-MS/MS with Isotopes Dilution / 中国药房
China Pharmacy
;
(12): 235-239, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-816728
ABSTRACT
OBJECTIVE: To establish a method for determination of fluconazole concentration in human plasma. METHODS: UPLC-MS/MS method was adopted to determine plasma after precipitated with acetonitrile. Using isotope fluconazole-d4 as internal standard, the determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% formic acid-acetonitrile (gradient elution) at the flow rate of 0.3 mL/min. The column temperature was 40 ℃, and the sample size was 3 μL. ESI was used for positive ion scanning by multiple reaction monitoring mode. The ion pairs for quantitative analysis were m/z 307.1→220.0 (fluconazole) and m/z 311.1→223.0 (internal standard). RESULTS: The linear range of fluconazole was 10-5 000 ng/mL (r=0.998 1). The limits of quantitation was 10 ng/mL. RSDs of intra-day and inter-day were less than 8%; accuracy ranged 95.8%-106.7%. The extraction recovery ranged 97.3%-107.3% (RSD<5.0%, n=6), and matrix effect, dilution effect and residual effect didn’t influence quantitative analysis of the substance to be measured. CONCLUSIONS: The method is simple, rapid, specific and accurate, which can be used for therapeutic drug monitoring and pharmacokinetic study of fluconazole.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2019
Type:
Article
Similar
MEDLINE
...
LILACS
LIS