Correlation of sperm DNA fragmentation index with semen parameters / 中华男科学杂志
National Journal of Andrology
;
(12): 129-134, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-816848
ABSTRACT
Objective@#To explore the relationship of sperm DNA fragmenation index (DFI) with semen parameters and assess its application value in the evaluation of semen quality.@*METHODS@#A total of 9 694 semen samples were collected and examined for sperm DFI and high DNA stainability (HDS) by flow cytometry-assisted sperm chromatin structure analysis (SCSA). According to the WHO Laboratory Manual for the Examination and Processing of Human Semen (5th Ed), the samples were divided into a normal group and abnormal groups A (sperm concentration [SC] [11.3-14.0] ×10⁶/ml, total sperm motility [TSM] 30%-39%, progressively motile sperm [PMS] 24%-31%), B (SC [7.5-11.2] ×10⁶/ml, TSM 20%-29%, PMS 16%-23%), C (SC [3.8-7.4] ×10⁶/ml, TSM 10%-,19% PMS 8%-15%) and D (SC [0-3.7]×10⁶/ml, TSM 0-9%, PMS 0-7%), and also into three sperm DFI groups (DFI 30%). The correlation between sperm DFI and seminal parameters was analyzed by Pearson correlation and multiple linear regression analyses.@*RESULTS@#DFI was dramatically lower in the normal than in the abnormal groups (P < 0.01), and increased in proportion to the decrease of semen parameters in the abnormal groups A, B, C and D (P < 0.01). Pearson correlation analysis showed that DFI was correlated positively with age (r = 0.15, P < 0.01), abstinence time (r = 0.10, P < 0.01), semen volume (r = 0.05, P < 0.01) and HDS (r = 0.15, P < 0.01), but negatively with semen pH (r = -0.06, P < 0.01), SC (r = -0.27, P < 0.01), TSM (r = -0.53, P < 0.01), PMS (r = -0.52, P < 0.01) and morphologically normal sperm (r = -0.16, P < 0.01). Multiple linear regression analysis revealed that TSM, SC, age, abstinence time and semen pH were five important variables associated with DFI, with standardized regression coefficients of -0.47, -0.19, 0.12, 0.07, and -0.04, respectively (all P < 0.01).@*CONCLUSIONS@#There is a moderate correlation between sperm DFI and semen parameters, which can be used synergistically for the assessment of semen quality.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
National Journal of Andrology
Year:
2019
Type:
Article
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