Study on Inhibitory Effect of Modified Maimendong Decoction Combined with Cisplatin on Lewis Lung Cancer Transplantation Model Mice and Its Mechanism / 中国药房
China Pharmacy
;
(12): 1226-1230, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-816968
ABSTRACT
OBJECTIVE: To study the inhibitory effect of modified Maimendong decoction combined with cisplatin on Lewis lung cancer transplantation model mice, and to explore its potential mechanism. METHODS: Lewis lung cancer transplantation mice model was induced via subaxillary inoculation of Lewis lung cancer cells. 60 mice were randomly divided into model group (normal saline, once a day, i.g.), cisplatin group (6 mg/kg, once a week, i.p.), modified Maimendong decoction group (20 g/kg. once a day, i.g.) and combination group (cisplatin 6 mg/kg, once a week, i.p.+modified Maimengdong decoction, once a day, i.g.), with 15 mice in each group. All mice were treated for consecutive 2 weeks. After medication, tumor weight and thymus index were detected; HE staining was used to observe the pathological change of tumor tissue. TUNEL was used to detect apoptotic index of tumor tissue. The protein expressions of Bcl-2 and Bax were detected by Western blot assay. RESULTS: Compared with model group, tumor weight and protein expression of Bcl-2 were decreased significantly in modified Maimengdong decoction, cisplatin group and combination group (P<0.05), and thymus index, tumor apoptotic index and the protein expression of Bax were increased significantly (P<0.05). Tumor weight and protein expression of Bcl-2 in combination group were significantly lower than modified Maimengdong decoction group and cisplatin group (P<0.05); thymus index, tumor apoptotic index and the protein expression of Bax were significantly higher than addition and subtraction of modified Maimengdong decoction group and cisplatin group (P<0.05). HE staining showed that the density of tumor cells was decreased to certain degree in modified Maimengdong decoction group, cisplatin group and combination group; the area of necrosis area in the combination group was significantly larger than in modified Maimendong decoction group and cisplatin group. CONCLUSIONS: Modified Maimendong decoction can inhibit the growth of Lewis lung cancer transplanted tumor in mice by down-regulating the protein expression of Bcl-2 and up-regulating the protein expression of Bax.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2019
Type:
Article
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