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Simultaneous Determination of Dehydrocorydine and Salvianolic Acid B in Shuangshen Tongguan Capsules by HPLC-dual Wavelength Switching Method / 中国药房
China Pharmacy ; (12): 886-889, 2019.
Article in Chinese | WPRIM | ID: wpr-817008
ABSTRACT
OBJECTIVE: To develop an method for determining the contents of dehydrocorydine and salvianolic acid B in Shuangshen tongguan capsules simultaneously. METHODS: The HPLC-dual wavelength switching method was used. The determination was performed on Waters symmetry C18 column with mobile phase consisted of acetonitrile-0.05% phosphoric acid solution (gradient elution) at the flow rate of 1.0 mL/min, the detection wavelength was 286 nm (salvianolic acid B) and 336 nm (dehydrocorydine). The column temperature was maintained at 25 ℃, and sample size was 10 μL. RESULTS: Under this condition, dehydrocorydaline and salvianolic acid B could be separated in baseline. The linear range of them were 0.157-1.259 μg and 0.391-3.131 μg (r=0.999 9). RSDs of precision, reproducibility and stability tests (within 24 h) were all lower than 2.00% (n=6-10). The average recovery rates were 101.61% and 102.85% (RSD=3.59% and 2.85%, n=6). CONCLUSIONS: Established HPLC-dual wavelength switching method can be used for simultaneous determination of dehydrocorydine and salvianolic acid B in Shuangshen tongguan capsules. The method is simple and rapid, and can be used for the quality control of Shuangshen tongguan capsule.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article