Establishment of HPLC Fingerprint of Pholidota chinensis and Its Cluster Analysis / 中国药房
China Pharmacy
;
(12): 1792-1795, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-817233
ABSTRACT
OBJECTIVE: To establish HPLC fingerprint of Pholidota chinensis and cluster analysis the samples, and to provide reference for quality evaluation of P. chinensis. METHODS: HPLC fingerprints of 16 batches of P. chinensis from Guangxi, Guangdong and Fujian were established according to 2012 edition of Similarity Evaluation System for Chromatographic Fingerprint of TCM [chromatographic column was Agilent SB-aq column with mobile phase consisted of acetonitrile-0.05% phosphoric acid solution (gradient elution), at the flow rate of 1.0 mL/min. The detection wavelength of 220 nm, the column temperature was 30 ℃ and the sample volume was 5 μL].The cluster analysis was carried out by using SPSS 22.0 statistical software. RESULTS: Accoding to the established HPLC fingerprint, 11 common peaks were indentified, and the common peak 1 was identified as gastrodin; the similarity of 16 batch of samples was greater than 0.9. 16 batches of medicinal materials could be divided into 2 groups. CONCLUSIONS: The established HPLC fingerprint and cluster analysis can provide reference for the quality control of P. chinensis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
China Pharmacy
Year:
2019
Type:
Article
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