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Effects and Mechanism of Water Extract and Ethanol Extract of Muskmelon Pedicel on the Proliferation ,Migration and Cloning Formation of Esophageal Carcinoma TE- 1 and EC- 1 Cells / 中国药房
China Pharmacy ; (12): 314-320, 2020.
Article in Chinese | WPRIM | ID: wpr-817336
ABSTRACT
OBJECTIVE:To study the effects and mechanism of water extract and ethanol extract of Muskmelon Pedicel on the proliferation,migration and cloning formation of esophageal carcinoma TE- 1 and EC- 1 cells. METHODS :TE-1 and EC- 1 cells were cultured in vitro ,and were treated with 0,1.562 5,3.125,6.25,12.5,25,50,100,200 μg/mL of water extract and ethanol extract of Muskmelon Pedicel (calulated by extract powder ). MTT assay was used to detect the growth inhibitory rate of TE- 1 and EC-1 cells,and calculate IC 50 of them. TE- 1 and EC- 1 cells were divided into TE- 1/EC-1 blank group ,TE-1/EC-1 Muskmelon Pedicel water extract group (IC50 as drug concentration ),and TE- 1/EC-1 Muskmelon Pedicel ethanol extract group (IC50 as drug concentration). The proliferation and migration of cells in each group were detected by real-time unlabeled cell analysis (RTCA), and cell proliferation and migration curves were drawn. The morphological changes of cells were observed under microscope ;soft agarose colony forming test was used to analyze the change of colony forming ability of cells in each group ,and the colony forming rate was calculated ;cell cycle and apoptosis rate of cells in each group were detected by flow cytometryWestern blotting assay was used to detect the relative expression of EGFR and PKC -α in cells in each group. RESULTS :IC50 of water extract of Muskmelon Pedicel were 49.24,76.38 μg/mL respectively for TE-1 and EC- 1 cells. Those of ethanol extract of Muskmelon Pedicel were 9.08,14.53 μ g/mL respectively for TE-1 and EC- 1 cells. The inhibition effect of water extract and ethanol extract of Muskmelon Pedicel on the cell proliferation were within 30 h. Δ 基金项目:河南省自然科学基金资助项目(No.162300410185) *博士研究生。研究方向:肿瘤中医方证。电话:0371-65676778。 The inhibition effect of water extract and ethanol extract of E-mail:zixiangning88@126.com Muskmelon Pedicel on the cell migration were within 60 h. # 通信作者 :教授,博士生导师 ,博士。研究方向 :肿瘤中医方 Compared with TE- 1/EC-1 blank group ,the number of cells 证。电话:0371-65676778。E-mail:sifc2000@hotmail.com was decreased significantly in administration groups , the ·314· China Pharmacy 2020Vol. 31 No. 3 中国药房 2020年第31卷第3期 structure of cell were sloop ,the cell structure was loose ,and most of the cell contour disappeared and became round. The formation rate of cell clone was decreased significantly (P<0.01). The percentage of G 2 phase cells increased significantly (P< 0.01),while that of G 1 and S phase cells decreased significantly (P<0.05). The apoptotic rate of cells increased significantly in early and late stage (P<0.05). Relative protein expression of EGFR and PKC- α were decreased significantly (P<0.01). Compared with TE- 1/EC-1 Muskmelon Pedicel water extract group ,formation rate of cell clone was decreased significantly in TE- 1/EC-1 Muskmelon Pedicel ethanol extract group (P<0.05);cell was increased significantly at G 2 phase(P<0.05);relative protein expression of EGFR and PKC- α were decreased significantly (P<0.01). The apoptotic rate of cells in early and late stage in TE- 1 Muskmelon Pedicel ethanol extract group was decreased significantly (P<0.05),the apoptotic rate of cells in early and late stage in EC- 1 Muskmelon Pedicel ethanol extract group was increased significantly (P<0.05). CONCLUSIONS :Water extract and ethanol extract of Muskmelon Pedicel could influence the proliferation ,migration and clone formation ability of TE- 1 and EC- 1 cell,promote cell apoptosis ,the mechanism of which may be associated with the down-regulation of EGFR and PKC-α protein.

Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Language: Chinese Journal: China Pharmacy Year: 2020 Type: Article