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Identification of 45 Kinds of Chemical Components of Forsythia suspensa by UPLC-Q-TOF-MS / 中国药房
China Pharmacy ; (12): 3067-3073, 2019.
Article in Chinese | WPRIM | ID: wpr-817443
ABSTRACT
OBJECTIVE: To identify chemical components of Forsythia suspense. METHODS: UPLC-Q-TOF-MS technology was used for the chemical components analysis of F. suspense. The determination was performed on ACQUITY UPLC BEH C18 column with mobile phase consisted of 0.1% formic acid aqueous solution (A)- 0.1% formic acid acetonitrile solution (B) with  gradient elution, at the flow rate of 0.3 mL/min; the column temperature was set at 30 ℃; the sample size was 5 μL. Positive and negative ions were detected by electrospray ionization. The temperature of the ion source was 550 ℃; the atomizing gas was N2; the atomizing gas and the auxiliary pressure were 379.2 kPa; the air curtain pressure was 241.3 kPa; the decluster voltage was 80 V/-80 V; the collision energy was 35 eV/-35 eV; the mass scanning range was 80-1 500 Da. Peakview 2.0 software was used to screen the target components by the first-order mass spectrometry, and calculate the high-resolution and accurate molecular weight of each component, compare with the reference spectrum and related literature, or calculate the elemental composition of fragment ions in the second-order mass spectrometry, analyze their decomposition pathways, then infer the structure of compounds. RESULTS & CONCLUSIONS: 45 kinds of compounds were identified from F. suspense,which included 7 phenylethanol glycosides,5 lignans,5 terpenes, 12 flavonoids,7 organic acids,2 phenols,2 quinones,2 glycosides and 3 other components. There were 19 compounds identified for the first time in F. suspense. The study provides a reference for the in-depth study of the pharmacodynamic substance basis of F. suspense and the rapid qualitative and quantitative analysis of the components.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Qualitative research Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Diagnostic study / Qualitative research Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article