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Study on Repair Effect of Artificial Isaria cicadae on Intestinal Mucosal Injury Induced by 5-Fluorouracil in Rats / 中国药房
China Pharmacy ; (12): 2973-2979, 2019.
Article in Chinese | WPRIM | ID: wpr-817479
ABSTRACT
OBJECTIVE: To study the repair effect of artificial Isaria cicadae on intestinal mucosal injury induced by 5-fluorouracil5-FU) in rats. METHODS: Forty SD rats were randomly divided into normal group (normal saline), model group (normal saline), artificial I. cicadae high-dose, medium-dose and low-dose groups (3.5, 1.75, 0.875 g/kg), with 8 rats in each group. Except for normal group, other groups were given intraperitoneal injection of 5-FU (0.25 g/10 mL) 30 mg/kg, once a day, for consecutive 5 days. At the same time, each group was given relevant medicine/normal saline intragastrically, once a day, for consecutive 8 d. After medication, body weight of rat was determined in each group. HE staining was used to observe the pathological change of small intestine. The levels of biobarrier-related factor [endotoxin (ET), D-lactic acid (D-LA)], immune barrier related factors (TNF-α, IFN-γ, sIgAIL-15G-CSF in serum and MPO, MDA in small intestine) and the levels of mechanical barrier related factors (connexin ZO-1 and Claudin-1) were detected. RESULTS: Compared with normal group, body weight of rats in model group was decreased significantly (P<0.01). Intestinal villus exfoliated obviously, the crypt structure was scattered, a large number of inflammatory cells gathered, and intestinal mucosa was seriously damaged. Serum levels of ET and D-LA, the levels of TNF-a, IFN-γ, MPO and G-CSF in serum, MDA level in small intestine were increased significantly (P<0.01). Serum levels of sIgA and IL-15 as well as the expressions of ZO-1 and Claudin-1 in small intestine were decreased significantly (P<0.05 or P<0.01). Compared with model group, body weight of rats in artificial I. cicadae high-dose group was increased significantly (P<0.01). The pathological changes of the small intestine of rats in each administration group were improved to varying degrees. The intestine morphology of artificial I. cicadae high-dose and medium-dose groups was close to that of the normal group. The levels of and ET, D-LA, TNF-α, IFN-γ, MPO, G-CSF in serum and the level of MDA in intestinal were decreased significantly (P<0.01). Serum levels of   sIgA and IL-15 in administration groups as well as the expressions of ZO-1 and Claudin-1 in intestinal tissue were increased significantly (P<0.01). CONCLUSIONS: Artificial I. cicadae can repair intestinal mucosal damage caused by 5-FU in respects of mechanical barrier, immune barrierbiological barrier.

Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article

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Full text: Available Index: WPRIM (Western Pacific) Type of study: Prognostic study Language: Chinese Journal: China Pharmacy Year: 2019 Type: Article