Protective Effects of Qizhi Tongluo Capsules and Their 9 Ingredients Decomposed Recipes for Promoting Blood Circulation and Removing Blood Stasis on Cerebral Ischemia-reperfusion Injury Model Rats / 中国药房

ABSTRACT

OBJECTIVE： To study the protective effects of Qizhi tongluo capsules and their 9 ingredients decomposed recipes for promoting blood circulation and removing blood stasis （PBCRBS） on cerebral ischemia-reperfusion injury model rats， and to lay a foundation for studying the active components of Qizhi tongluo capsules through decomposed recipes. METHODS： Totally 60 SD rats were randomly divided into sham operation group （0.5％ CMC-Na）， model group （0.5％ CMC-Na）， Qizhi tongluo capsules without the ingredients for PBCRBS group [0.389 g/（kg·d）]， the ingredients of Qizhi tongluo capsules for PBCRBS group [0.253  g/（kg·d）] and Qizhi tongluo capsules group [0.500 g/（kg·d）]， with 12 rats in each group. The rats were given relevant medicine intragastrically， once a day， for consecutive 14 d. 2 h after last medication， except for sham operation group， cerebral ischemia- reperfusion injury model was induced by suture method in each group. The neurological function score was measured according to Bederson score 2 h after ischemia and 24 h after reperfusion. The area of cerebral infarction was calculated by TTC staining. The contents of NO， MDA， LDH and SOD in cerebral tissue of rats were measured by biochemical method. The contents of IL-1β and TNF-α in cerebral tissue of rats were detected by ELISA. RESULTS： Compared with sham operation group， the neurological function score， the infarct area and the contents of NO， MDA， LDH， IL-1β and TNF-α in cerebral tissue were significantly increased in model group （P＜0.05）. The content of SOD in cerebral tissue were decreased significantly （P＜0.05）. Compared with model group， the neurological function score of rats was decreased significantly in each administration group （P＜0.05）； the area of cerebral infarction， the contents of NO， MDA， LDH， IL-1β and TNF-α in cerebral tissue were decreased significantly （P＜0.05）， while the content of SOD in cerebral tissue was increased significantly （P＜0.05）. Compared with Qizhi tongluo capsules group， the area of cerebral infarction， the contents of NO， MDA， LDH， IL-1β and TNF-α in cerebral tissue were increased in the ingredients of Qizhi tongluo capsules for PBCRBS group and Qizhi tongluo capsules without the ingredients for PBCRBS group （P＜0.05）， the content of SOD was decreased significantly （P＜0.05）. CONCLUSIONS： The formulation of Qizhi tongluo capsules and their 9 ingredients decomposed recipes for PBCRBS （without 9 ingredients for PBCRBS） have protective effects on cerebral ischemia-reperfusion injury in rats. Although the protective effects of decomposed recipes for PBCRBS is not as good as that of the whole prescription， the protective effects of whole formulation were decreased significantly. It shows that the drugs for PBCRBS play an important role in protecting the function of cerebral ischemia reperfusion injury. It is of certain significance to separate the ingredients for PBCRBS to clarify the effect of Qizhi tongluo capsules.