Over-expression of BMSC FoxM1 Attenuates LPS-induced Apoptosis of Alveolar Epithelial cells / 中山大学学报(医学科学版)
Journal of Sun Yat-sen University(Medical Sciences)
;
(6): 833-841, 2019.
Article
in Chinese
| WPRIM
| ID: wpr-817660
ABSTRACT
@#【Objective】To investigate whether bone marrow mesenchymal stem cells(BMSC)over-expressing FoxM1genecanattenuatelipopolysaccharide(LPS)-inducedapoptosisofalveolarepithelialcells,andexploreitspossi⁃ blemechanism.【Methods】SDratBMSCwereisolatedandculturedbywholebonemarrowadherencemethod.FoxM1 genewasoverexpressedinBMSCbylentiviraltransfection.TheexpressionofthetargetgeneFoxM1wasverifiedbyWestern blot.ApoptosisofA549cellswasmeasuredbyTUNELandflowcytometry.Andthemulti-factorlevelofsupernatantin BMSC-A549co-culturesystemwasdetectedbyMilliplexmethod.【Results】TUNELandflowcytometryconfirmedthat theapoptosisrateofA549inducedbyLPSdecreasedafterco-culturewithBMSCoverexpressingFoxM1,andthediffer⁃ encewasstatisticallysignificant(P <0.05).MilliplexassayshowedthatthelevelsofIL-13,IL-21,IL-23,MIP-1a, MIP-1bandinBMSCoverexpressing FoxM1 geneandA549co-culturesystemweresignificantlyincreased,whilethe MIP-3alevelissignificantlyreduced.【Conclusion】BMSCoverexpressingFoxM1genecanattenuateLPS-inducedapop⁃ tosisofalveolarepithelialcells.BMSCmayplayananti-apoptoticrolebychangingthelevelsofinflammation-related cytokinesreleasedbyA549cells.
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Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Journal of Sun Yat-sen University(Medical Sciences)
Year:
2019
Type:
Article
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