Mechanism of Cinnamaldehyde Promoting the Apoptosis of RL95-2 Cells in Endometrial Carcinoma / 中山大学学报(医学科学版)

ABSTRACT

@#【Objective】To investigate the effect of cinnamaldehyde on the apoptosis of RL95- 2 cell in endometrial carcinoma. 【Methods】 Endometrial carcinoma RL95- 2 cells were treated with cinnamaldehyde，and the proliferation activity and IC50 of endometrial carcinoma RL95-2 cells were detected by MTT colorimetry assay. Apoptotic morphology was observed after Hoechst 33258 staining. The percentage of apoptosis in RL95-2 cells was measured by flow cytometry. Western blot analysis was used to test the effect of cinnamaldehyde on the expression of Cleaved caspase- 3，caspase-3， NF-κB·p65，IL-6 and IGF-R in RL95-2 cells.【Results】Cinnamaldehyde can reduce the viability rate of endometrial cancer RL95- 2 cells，which is related to the treatment duration and concentration. Compared with the solvent control group， the apoptosis percentage of RL95- 2 cells in the cinnamaldehyde group （0.29， 0.59， 1.20 mg/mL） was significantly increased after 48 hours（P < 0.01），typical apoptotic bodies were found ，and the expression of Cleaved caspase-3 protein was significantly increased（P < 0.01），there was no significant change in the expression of Caspase 3 protein（P > 0.05），while the expression of NF- κB · p65，IL- 6 and IGF- R protein were significantly increased（P <0.05）.【Conclusion】Cinnamaldehyde can reduce the expression of NF-κB·p65，IL-6 and IGF-R proteins in RL95-2 cells and promote the apoptosis of RL95-2 cells，thus playing an anti-endometrial cancer role.