Curcumin reduces the invasion and migration abilities of papillary thyroid carcinoma TPC1 cells / 医学研究生学报

ABSTRACT

Objective Comparatively few studies are reported on the invasion and migration of papillary thyroid carcinoma (PTC) TPC1 cells. This study was to investigate the effects of curcumin on the invasion and migration abilities of TPC1 cells and its possible action mechanisms.MethodsWe treated TPC1 single cell suspension with curcumin at the concentrations of 0 (DMSO solvent), 10, 20, 40, 60, 80 and 100 μmol/L. At 24 and 48 hours after exposure, we examined the inhibitory effect of curcumin on the cells by CCK8 assays, detected the migration and invasion abilities of the TPC1 cells by Transwell and wound healing assay, and determined the gene and protein expressions Glut1 and MT1MMP by RTPCR and Western blot, respectively.ResultsThere were statistically significant differences in the cell viability among different groups of the TPC1 cells (P<0.05) as well as in the cell migration ability at 24 hours between any two groups of the cells treated with curcumin at 0 μmol/L (\[0.842±0.096\] mm), 10 μmol/L (\[0.911±0.049\] mm), 20 μmol/L (\[0.926±0.107\] mm) and 40 μmol/L (\[1.076±0.093\] mm) (P<0.05) and at 48 hours (P<0.05). Statistically significant differences were also observed between any two of the 0, 10, 20 and 40 μmol/L groups in the number of migrated cells (196, 142, 57, and 17/100x visual field) (P<0.05) as well in the protein expression of Glut1 (0.786±0.112, 0.518±0.106, 0.359±0.121, and 0.266±0.087) (P<0.05) and the mRNA and protein expressions of MT1MMP (P<0.05).ConclusionCurcumin can inhibit the invasion and migration of thyroid papillary carcinoma TPC1 cells, which may be associated with the decreased expressions of Glut1 and MT1MMP.