Icaritin promotes maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signal pathway / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
; (6): 571-577, 2017.
Article
in Zh
| WPRIM
| ID: wpr-819079
Responsible library:
WPRO
ABSTRACT
Objective: To investigate the effect of icaritin on maturation and mineralization of mouse osteoblast MC3T3-E1 cells and its mechanism. Methods: The cultured MC3T3-E1 cells were divided into blank control group, CXC chemokine receptor type 4 (CXCR4) inhibitor (AMD3100) group, icaritin group, and icaritin plus AMD3100 group. The expression of CXCR4, stromal cell-derived factor 1 (SDF-1) and osteogenesis-related genes and proteins were detected by real-time RT-PCR and Western blotting after drug treatment for 24 h. The alkaline phosphatase (ALP) activity was determined with ALP kit on d3 and d6; calcium nodules were detected by alizarin red staining after drug treatment for 14 d. Results: Real time RT-PCR showed that compared with the blank control group, relative expressions of CXCR4, SDF-1 and osteogenesis-related genes in icaritin group were significantly increased (PPCXCR4 gene was decreased (PPPPPConclusion: Icaritin may promote maturation and mineralization of mouse osteoblast MC3T3-E1 cells through CXCR4/SDF-1 signaling pathway.
Full text:
1
Index:
WPRIM
Main subject:
Osteoblasts
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Pharmacology
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Flavonoids
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Calcification, Physiologic
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Signal Transduction
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3T3 Cells
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Gene Expression Regulation, Developmental
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Receptors, CXCR4
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Cell Biology
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Chemokine CXCL12
Limits:
Animals
Language:
Zh
Journal:
Journal of Zhejiang University. Medical sciences
Year:
2017
Type:
Article