Advances in the clinical application of high-throughput sequencing in detecting microbiota in cirrhotic ascites, serum, and feces / 临床肝胆病杂志

ABSTRACT

Liver cirrhosis is a chronic progressive liver disease, and patients with liver cirrhosis are highly susceptible to infection. The changes in microbiota in ascites, serum, and feces are associated with the progression and complications of liver cirrhosis. However, effective bacterial culture cannot be performed for most patients due to low bacterial abundance in ascites and blood, and thus it is difficult to conduct a comprehensive analysis. The high-throughput and high-speed advantages of next-generation sequencing technique can be used for pathogen biology and clinical diagnosis, including pathogen detection and identification, strain typing, and microbiome studies, to help clinicians optimize the application of antimicrobials. At present, the next-generation sequencing technique gradually becomes mature, making it possible to analyze the whole genome of microbiota.