Detection of urinary antigens and their seroreactivity with serum of patients in Leishmania donovani infection
Asian Pacific Journal of Tropical Medicine
;
(12): 367-370, 2011.
Article
in English
| WPRIM
| ID: wpr-819505
ABSTRACT
OBJECTIVE@#To detect leishmanial antigens in pre and post treated urine of visceral leishmaniasis (VL) patients.@*METHODS@#Urine and serum sample from three VL patients were collected. Ammonium sulphate precipitation and purification of urine sample was done for proteins isolation. SDS PAGE of proteins was done followed by western blotting, with the patient's pre and post treatment serum.@*RESULTS@#Eight proteins of molecular weights 17 kDa, 25 kDa, 28 kDa, 42 kDa, 47 kDa, 54 kDa, 60 kDa and 85 kDa were detected in the urine of VL patients before treatment. After treatment with miltefosine, none of the above proteins was detected in urine samples. The western blot analysis with pre treatment serum confirmed the antigenicity of four urinary proteins of molecular weights 25 kDa, 28 kDa, 54 kDa and 60 kDa. The seropositivity with 25 kDa and 28 kDa antigens was negative with serum obtained after the completion of treatment.@*CONCLUSIONS@#In the context to unavailability of a prognostic tool, urinary leishmanial antigens may offer a better choice and may also be useful as immunoprophylactic candidates.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Urine
/
Blood
/
Leishmania donovani
/
Blotting, Western
/
Diagnosis
/
Allergy and Immunology
/
Leishmaniasis, Visceral
/
Molecular Weight
/
Antigens, Protozoan
Type of study:
Diagnostic study
Limits:
Adult
/
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Type:
Article
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