Inhibition of Pim-1 attenuates the proliferation and migration in nasopharyngeal carcinoma cells
Asian Pacific Journal of Tropical Medicine
; (12): 645-650, 2012.
Article
in En
| WPRIM
| ID: wpr-819603
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To explore the role of proto-oncogene Pim-1 in the proliferation and migration of nasopharyngeal carcinoma (NPC) cells.@*METHODS@#Pim-1 expressions in NPC cell lines CNE1, CNE1-GL, CNE-2Z and C666-1 were examined by RT-PCR, western blotting and immunoflucesence, respectively. After CNE1, CNE1-GL and C666-1 cells were treated with different concentrations of Pim-1 special inhibitor, quercetagetin, the cell viability, colony formation rate and migration ability were analyzed.@*RESULTS@#Pim-1 expression was negative in well-differentiated CNE1 cells, whereas expressed weakly positive in poor-differentiated CNE-2Z cells and strongly positive in undifferentiated C666-1 cells. Interestingly, CNE1-GL cells that derived from CNE1 transfected with an Epstein Barr virus latent membrane protein-1 over-expression plasmid displayed stronger expression of Pim-1. Treatment of CNE1-GL and C666-1 cells with quercetagetin significantly decreased the cell viability, colony formation rate and migration ability but not the CNE1 cells.@*CONCLUSIONS@#These findings suggest that Pim-1 overexpression contributes to NPC proliferation and migration, and targeting Pim-1 may be a potential treatment for anti-Pim-1-expressed NPCs.
Full text:
1
Index:
WPRIM
Main subject:
Pathology
/
Pharmacology
/
Carcinoma
/
Tumor Stem Cell Assay
/
Biomarkers, Tumor
/
Cell Movement
/
Cell Survival
/
Nasopharyngeal Neoplasms
/
Blotting, Western
/
Chromones
Limits:
Humans
Language:
En
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2012
Type:
Article