Metallo- β - lactamase producing nonfermentative gram-negative bacteria: an increasing clinical threat among hospitalized patients
Asian Pacific Journal of Tropical Medicine
;
(12): 718-721, 2012.
Article
in English
| WPRIM
| ID: wpr-819620
ABSTRACT
OBJECTIVE@#To detect and evaluate the various methods for metallo- β -lactamases (MBL) production in Pseudomonas aeruginosa (P. aeruginosa) and Acinetobacter species.@*METHODS@#A total of 109 P. aeruginosa and 85 Acinetobacter species were screened for imipenem resistance by Kirby-Bauer disc diffusion methods. Detection of MBL production was done by imipenem-EDTA combined disc test, double disc synergy test (DDST) and imipenem-EDTA MBL E test.@*RESULTS@#A total of 63 (57.8%) strains of P. aeruginosa and 46 (54.1%) strains of Acinetobacter spp. were found to be resistant to imipenem. Of the 63 imipenem resistant P. aeruginosa tested for MBL production, 44 (69.8%) were found to be positive and among 46 imipenem resistant Acinetobacter, 19 (41.3%) were shown to be the MBL producers.@*CONCLUSIONS@#Imipenem-EDTA combined disc test and MBL E test are equally effective for MBL detection in both P. aeruginosa and Acinetobacter spp., but given the cost-constraints, combined disc can be used as a convenient screening method in the clinical microbiology laboratory.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Pseudomonas aeruginosa
/
Pseudomonas Infections
/
Acinetobacter
/
Beta-Lactamases
/
Acinetobacter Infections
/
Imipenem
/
Cross Infection
/
Beta-Lactam Resistance
/
Drug Therapy
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2012
Type:
Article
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