Effect of bone marrow mesenchymal stem cells on the Smad expression of hepatic fibrosis rats
Asian Pacific Journal of Tropical Medicine
;
(12): 321-324, 2014.
Article
in English
| WPRIM
| ID: wpr-819679
ABSTRACT
OBJECTIVE@#To investigate the impact of bone marrow mesenchymal stem cells on Smad expression of hepatic fibrosis rats.@*METHODS@#A total of 48 adult female SD rats were randomly divided into three groups, normal control group (n=10), observation group (n=19) with liver fibrosis model rats injected with BMSCs cells; model group (n=19), with liver fibrosis model rats injected with physiological saline. Serum index, TGF-β1 and Smad expression were detected.@*RESULTS@#Type III procollagen, IV collagen, hyaluronic acid, laminin levels of observation group were significantly lower than those of model group (P<0.05). The content and expression of TGF-β1 in serum and liver tissue of observation group were significantly lower than those of model group(P<0.05). Compared with normal control group, the Smad3, Smad4 mRNA and protein expression of model group were significantly increased, the Smad7 mRNA and protein expression were significantly reduced (P<0.05). Compared with model group, Smad3, Smad4 mRNA and protein expression of observation group were significantly reduced, and Smad7 mRNA expression were significantly increased (P<0.05).@*CONCLUSIONS@#BMSCs can regulate Smad expression to some extent, and reduce the degree of liver fibrosis.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
General Surgery
/
Bone Marrow Cells
/
Transforming Growth Factor beta
/
Collagen
/
Laminin
/
Rats, Sprague-Dawley
/
Cell Biology
/
Mesenchymal Stem Cell Transplantation
/
Smad Proteins
Type of study:
Prognostic study
Limits:
Animals
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2014
Type:
Article
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