Multiplex real-time RT-PCR for detecting chikungunya virus and dengue virus
Asian Pacific Journal of Tropical Medicine
; (12): 342-346, 2012.
Article
in En
| WPRIM
| ID: wpr-819771
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To develop diagnostic test for detection chikungunya virus (CHIKV and Dengue virus (DENV) infection.@*METHODS@#We have performed a rapid, accurate laboratory confirmative method to simultaneously detect, quantify and differentiate CHIKV and DENV infection by single-step multiplex real-time RT-PCR.@*RESULTS@#The assay's sensitivity was 97.65%, specificity was 92.59% and accuracy was 95.82% when compared to conventional RT-PCR. Additionally, there was no cross-reaction between CHIKV, DENV, Japanese encephalitis virus, hepatitis C, hepatitis A or hepatitis E virus.@*CONCLUSIONS@#This rapid and reliable assay provides a means for simultaneous early diagnosis of CHIKV and DENV in a single-step reaction.
Full text:
1
Index:
WPRIM
Main subject:
DNA Probes
/
Chikungunya virus
/
Sensitivity and Specificity
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Alphavirus Infections
/
Nucleic Acid Amplification Techniques
/
Dengue
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Dengue Virus
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Diagnosis
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Real-Time Polymerase Chain Reaction
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Chikungunya Fever
Type of study:
Diagnostic_studies
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Screening_studies
Limits:
Female
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Humans
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Male
Language:
En
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2012
Type:
Article