Macrophage inflammatory protein-1alpha mediates matrix metalloproteinase-9 enhancement in human adherent monocytes fed with malarial pigment
Asian Pacific Journal of Tropical Medicine
;
(12): 925-930, 2011.
Article
in English
| WPRIM
| ID: wpr-819852
ABSTRACT
OBJECTIVE@#To investigate the role of macrophage inflammatory protein-1alpha (MIP-1alpha) in the detrimental enhancement of matrix metalloproteinase-9 (MMP-9) expression, release and activity induced by phagocytosis of malarial pigment (haemozoin, HZ) in human monocytes.@*METHODS@#Human adherent monocytes were unfed/fed with native HZ for 2 h. After 24 hours, MIP-1alpha production was evaluated by ELISA in cell supernatants. Alternatively, HZ-unfed/fed monocytes were treated in presence/absence of anti-human MIP-1alpha blocking antibodies or recombinant human MIP-1alpha for 15 h (RNA studies) or 24 h (protein studies); therefore, MMP-9 mRNA expression was evaluated in cell lysates by Real Time RT-PCR, whereas proMMP-9 and active MMP-9 protein release were measured in cell supernatants by Western blotting and gelatin zymography.@*RESULTS@#Phagocytosis of HZ by human monocytes increased production of MIP-1 alpha, mRNA expression of MMP-9 and protein release of proMMP-9 and active MMP-9. All the HZ-enhancing effects on MMP-9 were abrogated by anti-human MIP-1alpha blocking antibodies and mimicked by recombinant human MIP-1alpha.@*CONCLUSIONS@#The present work suggests a role for MIP-1alpha in the HZ-dependent enhancement of MMP-9 expression, release and activity observed in human monocytes, highlighting new detrimental effects of HZ-triggered proinflammatory response by phagocytic cells in falciparum malaria.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Phagocytosis
/
Pharmacology
/
Enzyme-Linked Immunosorbent Assay
/
Monocytes
/
Gene Expression Regulation, Enzymologic
/
Up-Regulation
/
Blotting, Western
/
Malaria, Falciparum
/
Matrix Metalloproteinase 9
/
Drug Therapy
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Type:
Article
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