Transfection of bone marrow mesenchymal stem cells using green fluorescence protein labeled hVEGF165 recombinant plasmid mediated by liposome

ABSTRACT

OBJECTIVE@#To study the role of bone marrow mesenchymal stem cells (BMSCs) in construction of vascularized engineered tissue.@*METHODS@#hVEGF165 was amplified via RT-PCR before recombinant with pShuttle- green fluorescence protein;green fluorescent protein (GFP)-CMV. Then the recombinant shuttle plasmid was transfected into BMSCs with Lipofectamine(TM) 2000 for packaging and amplifying. hVEGF165 mRNA expression in BMSCs cells was tested.@*RESULTS@#The sequence of hVEGF165 in pShuttle-GFP-hVEGF165 plasmid was confirmed by double-enzyme cleavage method and sequencing. hVEGF165 was highly expressed in BMSCs.@*CONCLUSIONS@#The GFP/hVEGF165 recombinant plasmid vector was constructed successfully and expressed effectively in host cells, which may be helpful for discussing the possibility of the application of VEGF165-BMSCs in tissue engineering and ischemic disease cure.