SIRT3 expression in hepatocellular carcinoma and its impact on proliferation and invasion of hepatoma cells
Asian Pacific Journal of Tropical Medicine
;
(12): 649-652, 2013.
Article
in English
| WPRIM
| ID: wpr-819988
ABSTRACT
OBJECTIVE@#To observe expression of SIRT3 in normal liver tissue, cirrhotic tissue and hepatocellular carcinoma (HCC) tissues, and to explore the significance of SIRT3 in primary HCC.@*METHODS@#SIRT3 expression was detected in 10 normal cases, 30 cases with, 30 HCC cases by immunohistochemical and Western-blotting method.@*RESULTS@#Immunohistochemical assay showed that the SIRT3 positive expression rates were 100.0% (10/10), 96.7% (29/30) and 60.0% (18/30), respectively in normal group, paracancer group and HCC group. And the SIRT3 expression in HCC group was significantly lower than in normal group and paracancer group (P<0.05). Western-blotting showed the SIRT3 expression in cancer tissue was 0.29±0.07, significantly lower than that in paracancer group and normal group (P<0.05). SIRT3 expression was related to the differentiation degree and portal vein tumor thrombus (P<0.05).@*CONCLUSIONS@#Abnormal expression of SIRT3 is closely related to the biological behavior of primary HCC.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Physiology
/
Immunohistochemistry
/
Carcinoma, Hepatocellular
/
Hepatocytes
/
Cell Proliferation
/
Sirtuin 3
/
Liver
/
Liver Cirrhosis
/
Liver Neoplasms
Limits:
Adult
/
Aged
/
Female
/
Humans
/
Male
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2013
Type:
Article
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