Influence of ellagic acid on prostate cancer cell proliferation: a caspase-dependent pathway
Asian Pacific Journal of Tropical Medicine
;
(12): 550-555, 2011.
Article
in English
| WPRIM
| ID: wpr-820097
ABSTRACT
OBJECTIVE@#To evaluate the effect of allagic acid treatment on the cell viability of human prostate cancer cells.@*METHODS@#Ellagic acid (10-100 mol/L) treatment (48 h) of human prostate carcinoma PC3 cells was found to result in a dose-dependent inhibition of cell growth and apoptosis of PC3 cells as assessed by MTT assay, western blotting, flow cytometry and confocal microscopy.@*RESULTS@#We observed that ellagic acid treatment of PC3 cells resulted in a dose dependent inhibition of cell growth/cell viability. This ellagic acid caused cell growth inhibition was found to be accompanied by induction of apoptosis, as assessed by the cleavage of poly (ADP-ribose) polymerase (PARP) and morphological changes. Further, induction of apoptosis accompanied a decrease in the levels of antiapoptotic protein Bcl-2 and increase in proapoptotic protein Bax, thus shifting the Bax Bcl-2 ratio in favor of apoptosis. Ellagic acid treatment of PC3 cells was also found to result in significant activation of caspases, as shown by the dose dependent decrease in the protein expression of procaspase-3, -6, -8 and -9. This ellagic acid-mediated induction of apoptosis was significantly (80%-90%) inhibited by the caspase inhibitor N-benzyloxycarbonyl-Val-Ala-Asp (OMe)-fluoromethylketone (Z-VAD-FMK). Thus these data suggested an essential role of caspases in ellagic acid-mediated apoptosis of PC3 cells.@*CONCLUSIONS@#It is tempting to suggest that consumption of tropical pigmented fruits and vegetables could be an effective strategy to combat prostate cancer.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pharmacology
/
Prostatic Neoplasms
/
Signal Transduction
/
Gene Expression Regulation, Neoplastic
/
Cell Survival
/
Poly(ADP-ribose) Polymerases
/
Apoptosis
/
Proto-Oncogene Proteins c-bcl-2
/
Caspases
/
Cell Line, Tumor
Limits:
Humans
/
Male
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Type:
Article
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