Pokemon reduces Bcl-2 expression through NF-κ Bp65: A possible mechanism of hepatocellular carcinoma
Asian Pacific Journal of Tropical Medicine
;
(12): 492-497, 2011.
Article
in English
| WPRIM
| ID: wpr-820106
ABSTRACT
OBJECTIVE@#To investigate the relationship among Pokemon, NF-κ B p65 and Bcl-2 in hepatoma cells.@*METHODS@#HCC cell HepG2, SMMC7721 and human fetal liver cell line LO2 cells were used, and expression of Pokemon, NF-κ B p65 and Bcl-2 in three cells were detected by real-time PCR and western blot. Then siRNA of Pokemon was applied to inhibit the expression of Pokemon and NF-κ B p65 and apoptotic rate was determined by flow cytometric analysis.@*RESULTS@#Expressions of Pokemon, NF-κ B p65 and Bcl-2 in human hepatoma cell HepG2, SMMC7721 expression were significantly higher than those in human embryonic stem cells LO2. siRNA of Pokemon inhibited the expression of Pokemon, NF-κ B p65 and Bcl-2 in liver cancer cells, and significantly increased apoptosis of liver cells. While siRNA of NF-κ B p65 inhibited the expression of NF-κ B p65 and Bcl-2, but Pokemon expression in hepatoma cells had no significant change.@*CONCLUSIONS@#The proto-oncogene Pokemon can inhibit P14ARF by specific transcription regulation of cell cycle and can induce tumors. In addition, Pokemon can regulate NF-κ B p65 through the expression of apoptosis repressor, and promote the development of liver cancer. It suggests signal network in the liver include the regulation of new non-classical NF-κ B regulatory pathway.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Transcription Factors
/
RNA, Messenger
/
Apoptosis
/
Carcinoma, Hepatocellular
/
Proto-Oncogene Proteins c-bcl-2
/
RNA, Small Interfering
/
Cell Line, Tumor
/
DNA-Binding Proteins
/
Transcription Factor RelA
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2011
Type:
Article
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