Effect and mechanism of miR-34a on proliferation, apoptosis and invasion of laryngeal carcinoma cells
Asian Pacific Journal of Tropical Medicine
;
(12): 494-498, 2016.
Article
in English
| WPRIM
| ID: wpr-820237
ABSTRACT
OBJECTIVE@#To discuss the effect and mechanism of miR-34a on the proliferation, apoptosis and invasion of laryngeal carcinoma cells.@*METHODS@#The laryngeal squamous carcinoma Hep2 cells were transiently transfected with miR-34a mimics and miR-34a NC. The MTT, colony-forming assay, Hoechst staining and AnnexinV-PI double staining flow cytometry were employed to detect the effect of miR-34a on the viability and apoptosis of laryngeal squamous carcinoma Hep2 cells; Transwell assay to defect the effect of miR-34a on the migration and invasion of laryngeal squamous carcinoma Hep2 cells; western blot and RT-PCR assay to defect the effect of miR-34a mimics on the expression of survivin and Ki-67 mRNA in laryngeal squamous carcinoma Hep2 cells.@*RESULTS@#Compared with miR-34a NC group, the cell viability in miR-34 mimics group was significantly decreased (P < 0.01), the cell apoptosis rate was significantly increased (P < 0.01), the abilities of cell migration and invasion were significantly reduced (P < 0.01) and the expression of survivin and Ki-67 mRNA was significantly decreased (P < 0.01).@*CONCLUSIONS@#The increased expression of miR-34a can induce the apoptosis of Hep2 laryngeal carcinoma cells and inhibit the cell proliferation and invasion, which is related to the down-regulated expression of survivin and Ki-67.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2016
Type:
Article
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