Antioxidant activity of newly discovered lineage of marine actinobacteria
Asian Pacific Journal of Tropical Medicine
;
(12): 325-332, 2013.
Article
in English
| WPRIM
| ID: wpr-820518
ABSTRACT
OBJECTIVE@#To investigate the antioxidant activity of marine actinobacteria.@*METHODS@#The content of total phenolics, the level of antioxidant potential by DPPH radical scavenging activity, metal chelating activity, FRAP method, β carotene assay and NO scavenging activity in extract were determined.@*RESULTS@#In all the methods the extract exhibited good scavenging activity except NO scavenging activity. The IC(50) values of marine actinobacteria extract on DPPH radical were found to be 41.09 μg/mL. The zone of color retention was 12 mm in β-carotene bleaching assay. DNA protective efficiency of the extracts was also studied using UV-photolysed H(2)O(2)-driven oxidative damage to pBR322. HPLC analysis identified some of the major phenolic compounds in extracts, which might be responsible for the antioxidant potential and cyto-protection. It showed a 100% cytotoxic effect in brine shrimp lethality assay within 10 mins. The novel actinobacteria was identified as Streptomyces LK-3 (JF710608) through 16S rDNA Sequencing.@*CONCLUSIONS@#The results obtained suggest that the extracts bear anti-cancer metabolites and could be considered as a potential source for anti-cancer drug development.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Oxidation-Reduction
/
Phenols
/
Picrates
/
Artemia
/
Streptomyces
/
Biphenyl Compounds
/
DNA Damage
/
Microbial Sensitivity Tests
/
Chelating Agents
/
Chemistry
Limits:
Animals
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2013
Type:
Article
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