Expression change of TNF-α in myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopolysaccharide
Asian Pacific Journal of Tropical Medicine
;
(12): 300-304, 2013.
Article
in English
| WPRIM
| ID: wpr-820522
ABSTRACT
OBJECTIVE@#To investigate the expression characteristics of TNF-α in myocardium and hepatic tissue of rats with compound stress of hyperthermia and lipopolysaccharide (LPS).@*METHODS@#Male SPF Wistar rats were randomly divided into room temperature+physiological saline group (Group C), hyperthermia+physiological saline group (Group H), room temperature+LPS group (Group L) and hyperthermia+LPS group (Group HL). The rats were put in simulated climate cabin. Group HL and Group H were exposed in the environment at a dry bulb temperature (TDB) of (35.0±0.5) °C, while Group L and Group C were exposed in the environment at a TDB of (26.0±0.5) °C. The rats in Group HL and Group L were given tail intravenous injection of LPS 10 mg/kg, while the rats in Group H and Group C were given tail intravenous injection of 9 g/L NaCl 10 mL/kg. After the stress, immunohistochemical SABC staining method was used to detect the expression characteristics of TNF-α in myocardium and hepatic tissue of rats, and those rats were given routine pathological examinations.@*RESULTS@#The expression of TNF-α in myocardium and hepatic tissue in Group HL was enhanced remarkably, and the tissue damages of Group HL were severest.@*CONCLUSIONS@#The cardiotoxicity and hepatotoxicity caused by compound stress of hyperthermia and LPS is closely related to the expression of TNF-α.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Physiology
/
Stress, Physiological
/
Immunohistochemistry
/
Random Allocation
/
Lipopolysaccharides
/
Tumor Necrosis Factor-alpha
/
Rats, Wistar
/
Systemic Inflammatory Response Syndrome
/
Fever
/
Arterial Pressure
Limits:
Animals
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2013
Type:
Article
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