Design of a multiplex PCR method for detection of toxigenic-pathogenic in Vibrio cholerae
Asian Pacific Journal of Tropical Medicine
;
(12): 115-118, 2013.
Article
in English
| WPRIM
| ID: wpr-820558
ABSTRACT
OBJECTIVE@#To study virulence and regulatory genes (hlyA, ctxB, tcpI) in clinical strains of Vibrio cholerae (V. cholerae), simultaneously.@*METHODS@#Three important genes, tcpI, hlyA and ctxB were used for detection of toxigenic and pathogenic V. cholera by chain reaction assay method.@*RESULTS@#According to the results of the PCR, the incidence of hlyA, tcpI, and ctxB genes in clinical isolates was obtained as 94.7% (72 sample), 90.8% (69 sample), and 92.1% (70 sample), respectively. Five strains possessed all genes except ctxB, six strains possessed all genes except tcpI, four strains possessed all genes except hlyA, one strain possessed only hlyA and 60 strains contained a combination of three genes, Including hlyA, ctxB and tcpI.@*CONCLUSIONS@#Result show that this method could be reliable to detect toxigenic-pathogenic strains of V. cholerae in Iran.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Repressor Proteins
/
Bacterial Proteins
/
Vibrio cholerae
/
Virulence
/
DNA, Bacterial
/
Cholera
/
Sensitivity and Specificity
/
Classification
/
Feces
/
Multiplex Polymerase Chain Reaction
Type of study:
Diagnostic study
Limits:
Humans
Country/Region as subject:
Asia
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2013
Type:
Article
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