Influence of Ginkgo biloba extract on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma of lacrimal gland
Asian Pacific Journal of Tropical Medicine
;
(12): 897-900, 2012.
Article
in English
| WPRIM
| ID: wpr-820601
ABSTRACT
OBJECTIVE@#To explore the influence of extract of Ginkgo biloba (EGB) on the proliferation, apoptosis of ACC-2 cell and Survivin gene expression in adenoid cystic carcinoma (ACC) of lacrimal gland.@*METHODS@#ACC-2 cell in human with ACC of lacrimal gland was in vitro cultured. MTT method was used for cell proliferation detection. Annexin V/PI double-staining flow cytometer was used to detect cell apoptosis and cell cycle. Survivin gene expression was analyzed by RT-PCR and Western blotting.@*RESULTS@#EGB had inhibitory effect on the proliferation of ACC-2 cell with significant dose-effect relationship, and there was statistical difference when compared with the control group (P<0.01). The inhibitory concentration 50 % (IC(50)) is 88 mg/L. The flow cytometer test indicated that EGB can gradually increase ACC-2 cell in G(0)-G(1) stage and decrease it in G(2)-M and S stage. With the increase of dose, the apoptosis rate of ACC-2 cell was obviously increased (P<0.05 or P<0.01). EGB had certain inhibitory effect on Survivin gene expression of ACC-2 cell, and Survivin gene expression was decreased with the increasing of the EGB concentration (P<0.01).@*CONCLUSIONS@#EGB can effectively inhibit Survivin gene expression of ACC-2 cell in human with ACC of lacrimal gland, induce the apoptosis of ACC-2 cell and inhibit tumor cell proliferation.
Full text:
Available
Index:
WPRIM (Western Pacific)
Main subject:
Pathology
/
Pharmacology
/
Staining and Labeling
/
Tetrazolium Salts
/
Thiazoles
/
Plant Extracts
/
Gene Expression
/
Cell Survival
/
Chemistry
/
Apoptosis
Limits:
Humans
Language:
English
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2012
Type:
Article
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