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Molecular determination and genotyping of Cryptosporidium spp. in fecal and respiratory samples of industrial poultry in Iran
Article in En | WPRIM | ID: wpr-820690
Responsible library: WPRO
ABSTRACT
OBJECTIVE@#To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran.@*METHODS@#A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing.@*RESULTS@#From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi.@*CONCLUSIONS@#In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.
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Full text: 1 Index: WPRIM Main subject: Parasitology / Poultry Diseases / Trachea / Chickens / Polymerase Chain Reaction / Epidemiology / Prevalence / DNA, Protozoan / Cryptosporidiosis / Cryptosporidium Type of study: Prevalence_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: Asian Pacific Journal of Tropical Medicine Year: 2014 Type: Article
Full text: 1 Index: WPRIM Main subject: Parasitology / Poultry Diseases / Trachea / Chickens / Polymerase Chain Reaction / Epidemiology / Prevalence / DNA, Protozoan / Cryptosporidiosis / Cryptosporidium Type of study: Prevalence_studies Limits: Animals Country/Region as subject: Asia Language: En Journal: Asian Pacific Journal of Tropical Medicine Year: 2014 Type: Article