Molecular determination and genotyping of Cryptosporidium spp. in fecal and respiratory samples of industrial poultry in Iran
Asian Pacific Journal of Tropical Medicine
; (12): 517-520, 2014.
Article
in En
| WPRIM
| ID: wpr-820690
Responsible library:
WPRO
ABSTRACT
OBJECTIVE@#To identify the genotypes of prevalent Cryptosporidia in broiler chickens in Lorestan province, Iran.@*METHODS@#A total of 1 000 fecal and 1 000 trachea samples were collected from chickens. Smears from both fecal and tracheal samples were stained with modified Ziehl-Neelsen method and nested PCR-RFLP according to amplification of 18S rRNA gene using Ssp I and Vsp I restriction enzymes and DNA sequencing.@*RESULTS@#From the examined chickens 0.7% was positive for Cryptosporidium. Infection was present in 0.5% fecal samples and also in 0.5% trachea. Only 0.3% of simultaneous infections in fecal and tracheal samples were observed. Nested PCR of our isolates demonstrated Cryptosporidium baileyi.@*CONCLUSIONS@#In our work, low rate of Cryptosporidium baileyi infection was detected, but in critical situations and our poor management circumstances, cryptosporidiosis occurs in serious feature especially in immune suppressed individuals.
Key words
Full text:
1
Index:
WPRIM
Main subject:
Parasitology
/
Poultry Diseases
/
Trachea
/
Chickens
/
Polymerase Chain Reaction
/
Epidemiology
/
Prevalence
/
DNA, Protozoan
/
Cryptosporidiosis
/
Cryptosporidium
Type of study:
Prevalence_studies
Limits:
Animals
Country/Region as subject:
Asia
Language:
En
Journal:
Asian Pacific Journal of Tropical Medicine
Year:
2014
Type:
Article