Effect of Tim-4 on invasion and migration of SiHa cervical cancer cell and its underlying mechanism / 中国肿瘤生物治疗杂志
Chinese Journal of Cancer Biotherapy
;
(6): 270-274, 2018.
Article
in Chinese
| WPRIM
| ID: wpr-821334
ABSTRACT
@#[Abstract] Objective:
To investigate the effect of Tim-4 on invasion and migration of cervical cancer cells and its underlying mechanisms. Methods:The expression levels of Tim-4 in cervical cancer cell lines Siha, Hela and cervical epithelial immortalized cell line H8 were detected by Real-time PCR. The Tim-4 lentiviral vector was transfected into Siha cell line. The over-expression of Tim-4 in Siha cell line was detected by fluorescence microscopy. The effects of Tim-4 on the invasion and migration of cervical cancer cell line were detected by Transwell and scratches methods. The changes of MMP2, MMP9, E-cadherin and N-cadherin in Siha cells were detected by Western blotting. Results:The expression of Tim-4 was higher in Siha and Hela cell lines compared to that in the H8 cell line. The Siha cell line burdening Tim-4 lentiviral vector was successfully constructed. Over-expression of Tim-4 significantly inhibited the migration and invasion of cervical cancer cell line, and affected the expression of MMP2, MMP9, N-cadherin and E-cadherin. Conclusion:Over-expression of Tim-4 promotes the invasion and migration by regulating the EMT transformation in cervical cell carcinoma.
Full text:
Available
Index:
WPRIM (Western Pacific)
Language:
Chinese
Journal:
Chinese Journal of Cancer Biotherapy
Year:
2018
Type:
Article
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