Optimization of Ultrasonic-assisted Ethanol- (NH4)2SO4 Aqueous Two-phase Extraction of Citrusinol from Desmo- dium caudatum by Box-Behnken Design-response Surface Methodology / 中国药房
China Pharmacy
;
(12): 1085-1090, 2020.
Article
in Chinese
| WPRIM
| ID: wpr-821498
ABSTRACT
OBJECTIVE:To optimize ultrasonic-assisted ethanol-(NH4)2SO4 aqueous two-phase extraction technology of citru- sinol from Desmodium caudatum . METHODS :Using the content of citrusinol as indexes ,with ethanol volume fraction , solid-liquid ratio ,(NH4)2SO4 addition amount ,ultrasonic time and ultrasonic temperature as factors ,based on the single factor tests,Box-Behnken design-response surface methodology was used to optimize the extraction technology of citrusinol. RESULTS : The optimized extraction technology of citrusinol included that ethanol volume fraction was 95.35%,the solid-liquid ratio was 1∶50.35 (g/mL),(NH4)2SO4 addition amount was 4.49 g,ultrasonic time was 48.7 min,ultrasonic temperature was 57.6 ℃. In 3 times of validation tests ,the extraction rates of citrusinol were 0.637 8,0.638 4,0.625 4 mg/g,respectively,which was close to predicted value(0.630 5 mg/g). CONCLUSIONS :The optimized ultrasonic-assisted ethanol- (NH4)2SO4 aqueous two-phase extraction technology is stable and feasible ,and can be used for the extraction of citrusinol from D. caudatum .
Full text:
Available
Index:
WPRIM (Western Pacific)
Type of study:
Prognostic study
Language:
Chinese
Journal:
China Pharmacy
Year:
2020
Type:
Article
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